Project Goals and Objectives
The proposed project will focus on the study of the existence of a
nodule-specific hypoxia-related regulation of genes and its putative implication in the SNF, using the model symbiotic system of
Medicago truncatula - Sinorhizobium meliloti.
The specific objectives of this project are:
- In silico identification of M. truncatula nodule-specific expressed and nodule-induced genes with special focus on the corresponding sugar transporters and glycolysis-related genes.
- Determination of the temporal and spatial expression of these genes during the different stages of M. truncatula nodule development, and comparison with their expression levels in non-symbiotic plant organs (for the nodule-induced genes).
- Examination of the subcellular localization of the corresponding encoded proteins in the nodule, by expressing protein hybrids with a reporter protein or tagged with an epitope.
- Determination of the substrate specificity and kinetic properties of the targeted sugar transporters.
- Analysis of the physiological role of the identified sugar transporters and glycolysis-related genes in the process of SNF, using reverse genetic approaches.
- Characterization of the proximal promoters of these genes by in silico analysis as well as by testing the function of the promoter (using a reporter protein) in plants, in order to determine known and new hypoxia responsive DNA elements.
- Identification of nodule-expressed transcription factors capable of recognizing these hypoxia-responsive DNA elements.